SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3)

Cat. No. GTX537371-23

Application	PLA
Reactivity	SARS Coronavirus 2
Package	5 test ($529)

Datasheet
Document

PRODUCT

Summary

The SARS-CoV-2 Membrane Protein Proximity Ligation Assay (PLA) Kit (Cy3) enables the detection of SARS-CoV-2 membrane protein in virus-infected tissue or infected/transfected cell block samples. The viral membrane protein is detected using two oligonucleotide-conjugated primary antibodies, called PLA probes. Only when both PLA probes bind to the membrane protein can the assay generate an amplified signal. This provides researchers with a highly specific and sensitive diagnostic tool.

Content

Item	Name	Quantity	Storage
A1	Blocking Solution, 10 mL	2 bottles	-20 ºC
A2	Antibody (Ab)-Oligo A, 40 µL	1 tube	-20 ºC
A3	Antibody (Ab)-Oligo B, 40 µL	1 tube	-20 ºC
A4	Ligase (400 U/µL), 10 µL	1 tube	-20 ºC
A5	5x Ligation Solution, 500 µL	1 tube	-20 ºC
A6	Polymerase (10 U/µL), 10 µL	1 tube	-20 ºC
A7	5x Amplification Solution, 500 µL	1 tube	-20 ºC
A8	Detection Probe (Cy3), 50 µL	1 tube	-20 ºC
B1	10x Probe Solution, 200 µL	1 tube	4°C or below
B2	20x Wash Buffer, 50 mL	2 bottles	4°C or below

APPLICATION

Application Note

Please see the protocol for detailed procedure.

Product Note

This PLA kit detects SARS-CoV-2 membrane protein.

PROPERTIES

Form

Liquid

Storage

Individual components are stored at -20ºC or 4ºC as indicated.

Conjugation

Cyanine3 (Cy3)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX537371-23 PLA Image

Detection of SARS-CoV-2 (COVID-19) membrane protein in a transfected HEK293T cell FFPE Cell Pellet Block (GTX435640) section using the SARS-CoV-2 Membrane Protein Proximity Ligation Assay Kit (Cy3) (GTX537371-23). PLA signals appear yellow and nuclei are blue.
A) Positive PLA reaction in transfected HEK293T cells.
B) Negative control with mock-transfected HEK293T cells.
C) Negative control using one non-specific PLA antibody probe in transfected HEK293T cells.

GTX537371-23 Image

REFERENCE

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REVIEW

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SDS
GTX537371-23_PLA_MSDS.pdf

Package

List Price ($)

5 test

$ 529

Qty