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SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Flow cytometry (FACS). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696

Cat No. GTX632696

Reference 6
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5A10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, FACS

Reactivity

 SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($379),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nsp8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Positive Control

NSP8-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The immunogen used to generate this antibody corresponds to SARS virus nsp8.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888329

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV nsp8 , SARS-coV-2 nsp8 , COVID-19 nsp8

Research Area

DATA IMAGES

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696

GTX632696 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Flow cytometry (FACS). GTX632696

GTX632696 FACS Image

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis.
Sample: Vero E6 cells infected with SARS-CoV-2.
Black: Uninfected Vero E6 cells was used as a control.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) dilution: 1:100.
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632696

GTX632696 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696

GTX632696 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:5000.

REFERENCE

Application Reference

Patrick Madden et al. Res Sq 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.
Application : IHC-Fr
Madden PJ et al. bioRxiv 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.
Application : IHC-Fr
Ter Ellen BM et al. Viruses 2021; 13 (7) Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air-Liquid Interface Cultured Human Primary Bronchial Epithelial Cells.
Application : FACS
Ciccosanti F et al. Antiviral Res 2021; 190 : 105064 Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection.
Application : WB
Kotaki T et al. Sci Rep 2021; 11 (1) : 2229 A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation.
Application : ICC/IF
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Submit a Reference

REVIEW

SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] Cat No. GTX632696

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2020 in Cell Death Dis. PMID: 32814759
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Flow cytometry (FACS). GTX632696
Date : Anonymous submitted on 14-Jul-2020
Score :
Application Tested : FACS
Sample Species : SARS-CoV-2
Sample : Vero E6 cells infected with SARS-CoV-2 USA-WA1/2020
Fixation : 4% formaldehyde in PBS
Permeabilization /Antigen retrieval : BD Cytofix/Cytoperm
Primary Antibody : 1:100, 25°C, 0.5Hr
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV, SARS-CoV-2
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations