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SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] used in Western Blot (WB). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] used in Western Blot (WB). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] used in Flow cytometry (FACS). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632696

Cat No. GTX632696

Host

Mouse

Clonality

Monoclonal

Clone Name

5A10

Isotype

IgG1

Application

WB, ICC/IF, FACS

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to SARS virus NS8.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV nsp8 , SARS-coV-2 nsp8 , COVID-19 nsp8

Research Area

DATA IMAGES

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] used in Western Blot (WB). GTX632696

GTX632696 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] used in Western Blot (WB). GTX632696

GTX632696 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:5000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] used in Flow cytometry (FACS). GTX632696

GTX632696 FACS Image

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis.
Sample: Vero E6 cells infected with SARS-CoV-2.
Black: Uninfected Vero E6 cells was used as a control.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) dilution: 1:100.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632696

GTX632696 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] Cat No. GTX632696

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-Jul-2020
Score :
Application Tested : FACS
Sample Species : SARS-CoV-2
Sample : Vero E6 cells infected with SARS-CoV-2 USA-WA1/2020
Fixation : 4% formaldehyde in PBS
Permeabilization /Antigen retrieval : BD Cytofix/Cytoperm
Primary Antibody : 1:100, 25°C, 0.5Hr
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV, SARS-CoV-2
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf