SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10]

Name: SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10]
Brand: GeneTex
SKU: GTX632696
Price: 329.00 USD
Rating: 5 (2 reviews)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Flow cytometry (FACS). GTX632696

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632696

Cat No. GTX632696

Reference 3

Host	Mouse
Clonality	Monoclonal
Clone Name	5A10
Isotype	IgG1
Application	WB, ICC/IF, FACS
Reactivity	SARS Coronavirus, SARS Coronavirus 2
Package	100 μl ($329), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) nsp8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to SARS virus nsp8.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888329

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV nsp8 , SARS-coV-2 nsp8 , COVID-19 nsp8

Research Area

DATA IMAGES

GTX632696 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.

GTX632696 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:5000.

GTX632696 FACS Image

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis.
Sample: Vero E6 cells infected with SARS-CoV-2.
Black: Uninfected Vero E6 cells was used as a control.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) dilution: 1:100.

GTX632696 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Ciccosanti F et al. Antiviral Res 2021; 190 : 105064 Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection.

Application : WB

Kotaki T et al. Sci Rep 2021; 11 (1) : 2229 A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation.

Application : ICC/IF

Cell Death Dis. 2020; 11 (8) : 656 On-target versus off-target effects of drugs inhibiting the replication of SARS-CoV-2.

Application : WB

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