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SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Immunoprecipitation (IP). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

Cat. No. GTX632269

Reference 38
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6H3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632269 / GTX135361 / GTX135357, Detection : GTX135357 / GTX632269
Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV Nucleocapsid (121-422 a.a.).

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888304

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135357 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Immunoprecipitation (IP). GTX632269

GTX632269 IP Image

Immunoprecipitation of SARS-CoV-2 NP transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269).
Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135361 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269

GTX632269 ICC/IF Image

ICC/IF analysis of VeroE6 cells over-expressing SARS-CoV Nucleocapsid protein using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269

GTX632269 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Red: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269

GTX632269 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269

GTX632269 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

WB analysis of SARS-CoV infected Vero E6 cells harvested at different time-points post infection (T = 0, 8, 16 and 24 h) using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Swain J et al. iScience 2023; 26 (8) : 107384 F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells.
McMahon A et al. PLoS Pathog 2023; 19 (6) : e1011484 High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization.
Carrell C et al. Anal Methods 2023; 15 (22) : 2721-2728 Capillary driven microfluidic sequential flow device for point-of-need ELISA: COVID-19 serology testing.
Williams DM et al. iScience 2023; 26 (7) : 107056 Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy.
Hou P et al. Autophagy 2023; 19 (2) : 551-569 The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication.
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REVIEW

SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] Cat. No. GTX632269

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Date : Anonymous submitted on 05-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV
Sample : Caco2 cells
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Swain J et al. iScience 2023; 26 (8):107384 F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells.
McMahon A et al. PLoS Pathog 2023; 19 (6):e1011484 High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization.
Carrell C et al. Anal Methods 2023; 15 (22):2721-2728 Capillary driven microfluidic sequential flow device for point-of-need ELISA: COVID-19 serology testing.
Williams DM et al. iScience 2023; 26 (7):107056 Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy.
Hou P et al. Autophagy 2023; 19 (2):551-569 The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication.
Tamai K et al. Cell Rep Methods 2022; 2 (10):100314 iPSC-derived mesenchymal cells that support alveolar organoid development.
Application : ICC/IF
Kumar CS et al. ACS Infect Dis 2022; 8 (10):2119-2132 Virus-Like Particles of SARS-CoV-2 as Virus Surrogates: Morphology, Immunogenicity, and Internalization in Neuronal Cells.
Yeh CT et al. Biomedicines 2022; 10 (11) Immunoglobulin Y Specific for SARS-CoV-2 Spike Protein Subunits Effectively Neutralizes SARS-CoV-2 Infectivity and Ameliorates Disease Manifestations In Vivo.
Pin-Hsuan Chen et al. Sens Actuators B Chem 2022; 357:131415 Saliva-based COVID-19 detection: A rapid antigen test of SARS-CoV-2 nucleocapsid protein using an electrical-double-layer gated field-effect transistor-based biosensing system.
Yasuo Ariumi et al. J Virol 2022; 96 (6):e0000222 Host Cellular RNA Helicases Regulate SARS-CoV-2 Infection.
Application : WB, ICC/IF, IP
Patrick Madden et al. Res Sq 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.
Application : IHC-Fr
Madden PJ et al. bioRxiv 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.
Application : IHC-Fr
Magalh?es AC et al. Pathogens 2022; 11 (3) InfectionCMA: A Cell MicroArray Approach for Efficient Biomarker Screening in In Vitro Infection Assays.
Application : IHC-P
Martin MDGM et al. Insights Imaging 2022; 13 (1):7 Postmortem brain 7T MRI with minimally invasive pathological correlation in deceased COVID-19 subjects.
Wen-Fang Tang et al. Biomed J 2021; 44 (3):293-303 Perilla (Perilla frutescens) leaf extract inhibits SARS-CoV-2 via direct virus inactivation.
Application : WB
S?bastien Lyonnais et al. Sci Rep 2021; 11 (1):11885 Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions.
Application : WB
Keiji Tanimoto et al. Sci Rep 2021; 11 (1):16629 Inhibiting SARS-CoV-2 infection in vitro by suppressing its receptor, angiotensin-converting enzyme 2, via aryl-hydrocarbon receptor signal.
Application : WB
Takeshi Yamamotoya et al. Sci Rep 2021; 11 (1):18581 Prolyl isomerase Pin1 plays an essential role in SARS-CoV-2 proliferation, indicating its possibility as a novel therapeutic target.
Application : WB
Duarte-Neto AN et al. Clin Infect Dis 2021; 73 (Suppl_5):S442-S453 Ultrasound-Guided Minimally Invasive Tissue Sampling: A Minimally Invasive Autopsy Strategy During the COVID-19 Pandemic in Brazil, 2020.
Serra AM et al. Braz J Infect Dis 2021; 25 (6):101651 SARS-CoV-2 identification in an acute appendicitis case: Acute abdomen as manifestation of Multisystem Inflammatory Syndrome in a child with COVID-19.
Application : IHC-P
Li X et al. Cell Mol Immunol 2021; SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy
Application : WB
Samper IC et al. ACS Sens 2021; 6 (11):4067-4075 Electrochemical Capillary-Flow Immunoassay for Detecting Anti-SARS-CoV-2 Nucleocapsid Protein Antibodies at the Point of Care.
Kim DH et al. Sci Rep 2021; 11 (1):21462 Hemin as a novel candidate for treating COVID-19 via heme oxygenase-1 induction.
Application : WB
Duarte-Neto AN et al. Andrology 2021; (Epub) Testicular pathology in fatal COVID-19: A descriptive autopsy study.
Application : IHC-P
Gopal V et al. ACS Appl Mater Interfaces 2021; 13 (26):30317-30325 Zinc-Embedded Polyamide Fabrics Inactivate SARS-CoV-2 and Influenza A Virus.
Application : WB
Sasaki M. et al. PLoS Pathog. 2021; 17 (1):e1009233 SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells.
Application : WB
Matsuura R et al. Viruses 2021; 13 (5) SARS-CoV-2 Disinfection of Air and Surface Contamination by TiO2 Photocatalyst-Mediated Damage to Viral Morphology, RNA, and Protein.
Application : WB
Ciccosanti F et al. Antiviral Res 2021; 190:105064 Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection.
Application : WB, ICC/IF
Mou H et al. PLoS Pathog 2021; 17 (4):e1009501 Mutations derived from horseshoe bat ACE2 orthologs enhance ACE2-Fc neutralization of SARS-CoV-2.
Application : ICC/IF
Di Domenico M et al. Diagnostics (Basel) 2021; 11 (4) Detection of SARS-COV-2 Proteins Using an ELISA Test.
Duarte-Neto AN et al. EClinicalMedicine 2021; 35:100850 An autopsy study of the spectrum of severe COVID-19 in children: From SARS to different phenotypes of MIS-C.
Application : IHC-P
Tang W.F. et al. Biomedical Journal 2021; (Epub) Perilla (Perilla frutescens) leaf extract inhibits SARS-CoV-2 via direct virus inactivation.
Application : WB
Li J et al. ACS Sens 2021; 6 (3):1270-1278 Microfluidic Magneto Immunosensor for Rapid, High Sensitivity Measurements of SARS-CoV-2 Nucleocapsid Protein in Serum.
Tanimoto K. et al. bioRxiv 2021; (Epub) Inhibiting SARS-CoV-2 infection in vitro by suppressing its receptor, angiotensin-converting enzyme 2, via aryl-hydrocarbon receptor signal.
Application : WB
Moustaqil M et al. Emerg Microbes Infect 2021; 10 (1):178-195 SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species.
Application : WB
Kotaki T et al. Sci Rep 2021; 11 (1):2229 A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation.
Application : ICC/IF
Gopal V et al. bioRxiv 2020; (Epub) Zinc-embedded fabrics inactivate SARS-CoV-2 and influenza A virus.
Application : WB
Zhang Y et al. Antiviral Res 2020; 185:104974 A bacterial artificial chromosome (BAC)-vectored noninfectious replicon of SARS-CoV-2.
Application : WB
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