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SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Western Blot (WB). GTX632602
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Western Blot (WB). GTX632602
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Western Blot (WB). GTX632602
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632602
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632602

Cat No. GTX632602

Host

Mouse

Clonality

Monoclonal

Clone Name

3C9

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Observed MW

15 kDa. The observed M.W. is based on the following publication. PMID: 15220404

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV ORF7a protein (16-111 a.a.).

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888320

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV ORF7a , SARS-coV-2 ORF7a , COVID-19 ORF7a

Research Area

DATA IMAGES

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Western Blot (WB). GTX632602

GTX632602 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Western Blot (WB). GTX632602

GTX632602 WB Image

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV ORF7a protein by western blot analysis. Non-transfected (-) and SARS-CoV ORF7a-transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 15220404

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Western Blot (WB). GTX632602

GTX632602 WB Image

WB analysis of SARS-CoV-infected Vero E6 cells harvested at different time points postinfection using GTX632602 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632602

GTX632602 ICC/IF Image

ICC/IF analysis of Vero E6 cells expressing ORF7a using GTX632602 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632602

GTX632602 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a stained by SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] Cat No. GTX632602

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV
Sample : Caco2 cells
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf