SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9]

Name: SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9]
Brand: GeneTex
SKU: GTX632602
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Western Blot (WB). GTX632602

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632602

Cat. No. GTX632602

Reference 4

Host	Mouse
Clonality	Monoclonal
Clone Name	3C9
Isotype	IgG1
Application	WB, ICC/IF
Reactivity	SARS Coronavirus, SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) ORF7a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Observed MW

15 kDa. The observed M.W. is based on the following publication. PMID: 15220404

Positive Control

ORF7a-transfected 293T cell

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV ORF7a protein (16-111 a.a.).

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888320

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV ORF7a , SARS-coV-2 ORF7a , COVID-19 ORF7a

Research Area

DATA IMAGES

GTX632602 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:1000.

GTX632602 WB Image

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV ORF7a protein by western blot analysis. Non-transfected (-) and SARS-CoV ORF7a-transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 15220404

GTX632602 WB Image

WB analysis of SARS-CoV-infected Vero E6 cells harvested at different time points postinfection using GTX632602 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].

GTX632602 ICC/IF Image

ICC/IF analysis of Vero E6 cells expressing ORF7a using GTX632602 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].

GTX632602 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a stained by SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (GTX632602) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Najla Arshad et al. bioRxiv 2022; SARS-CoV-2 accessory proteins ORF7a and ORF3a use distinct mechanisms to downregulate MHC-I surface expression.

Application : WB

Laura Martin-Sancho et al. Mol Cell 2021; Functional landscape of SARS-CoV-2 cellular restriction.

Application : WB

Ciccosanti F et al. Antiviral Res 2021; 190 : 105064 Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection.

Application : WB

Allan Sauvat et al. Cell Death Dis 2020; 11 (8) : 656 On-target versus off-target effects of drugs inhibiting the replication of SARS-CoV-2.

Application : WB

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