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SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunoprecipitation (IP). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

Cat No. GTX632269

Host

Mouse

Clonality

Monoclonal

Clone Name

6H3

Isotype

IgG1

Application

WB, ICC/IF, IP, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632269/GTX135361/GTX135357, Detection: GTX135357/GTX632269

Not tested in other applications.

Specificity/Sensitivity

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV Nucleocapsid (121-422 a.a.).

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135357 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunoprecipitation (IP). GTX632269

GTX632269 IP Image

Immunoprecipitation of SARS-CoV-2 NP transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269).
Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV Nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135361 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269

GTX632269 ICC/IF Image

ICC/IF analysis of VeroE6 cells over-expressing SARS-CoV Nucleocapsid protein using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

WB analysis of SARS-CoV infected Vero E6 cells harvested at different time-points post infection (T = 0, 8, 16 and 24 h) using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269

GTX632269 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269

GTX632269 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269

GTX632269 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Red: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] Cat No. GTX632269

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV
Sample : Caco2 cells
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf