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SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunoprecipitation (IP). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

Cat No. GTX632269

Host

Mouse

Clonality

Monoclonal

Clone Name

6H3

Isotype

IgG1

Application

WB, ICC/IF, IP, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632269/GTX135361/GTX135357, Detection: GTX135357/GTX632269

Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV Nucleocapsid (121-422 a.a.).

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888304

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135357 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunoprecipitation (IP). GTX632269

GTX632269 IP Image

Immunoprecipitation of SARS-CoV-2 NP transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269).
Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135361 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269

GTX632269 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

GTX632269 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269

GTX632269 ICC/IF Image

ICC/IF analysis of VeroE6 cells over-expressing SARS-CoV Nucleocapsid protein using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

WB analysis of SARS-CoV infected Vero E6 cells harvested at different time-points post infection (T = 0, 8, 16 and 24 h) using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269

GTX632269 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269

GTX632269 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Red: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

GTX632269 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] Cat No. GTX632269

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV
Sample : Caco2 cells
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf