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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunoprecipitation (IP). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Flow cytometry (FACS). GTX632604

Cat No. GTX632604

Host

Mouse

Clonality

Monoclonal

Clone Name

1A9

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IP, ELISA, sELISA

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture : GTX632604, Detection : GTX135360/ GTX135386

Not tested in other applications.

Specificity/Sensitivity

This antibody detects both SARS-CoV spike and SARS-CoV-2 spike proteins (S2 subunit). Our internal testing indicates no cross-reactivity with MERS-CoV spike protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV SΔ10 (within S2 domain) protein (1029-1192 a.a.).

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV Spike , 2019-nCoV Spike , COVID-19 Spike , SARS-CoV-2 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco-2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635672 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

GTX632604 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: BHK-21 cells transfected with full-length SARS-CoV-2 spike were fixed in 4% paraformaldehyde at RT for 30 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunoprecipitation (IP). GTX632604

GTX632604 IP Image

Immunoprecipitation of SARS-CoV-2 Spike transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) at 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

GTX632604 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 by immunofluorescent analysis.
Sample: Vero E6 cells were infected with SARS-CoV-2 (MOI of 1) and fixed with chilled methanol/acetone.
Green: SARS-CoV-2 infected Vero E6 cells stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Blue: Nuclei were counterstained with DAPI.

*From Zheng Z, et al. bioRxiv(2020). Shown under license agreement

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632604

GTX632604 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2-transfected COS-7 cells were fixed in 4% paraformaldehyde for 10 min at room temperature.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Blue: Nuclei were counterstained with DAPI.

*From Zheng Z, et al. bioRxiv(2020). Shown under license agreement

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135386 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in ELISA (ELISA). GTX632604

GTX632604 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135360 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604

GTX632604 IHC-P Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in IHC (Paraffin sections) (IHC-P). GTX632604

GTX632604 IHC-P Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Red: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Western Blot (WB). GTX632604

GTX632604 WB Image

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV Spike protein by western blot analysis. Non-transfected (-) and SARS-CoV Spike-transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] used in Flow cytometry (FACS). GTX632604

GTX632604 FACS Image

FACS analysis of 293T cells infected with recombinant vaccinia virus carrying the Spike gene using GTX632604 SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9].

REFERENCE

REVIEW

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] Cat No. GTX632604

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-Mar-2020
Score :
Sample Species : SARS-CoV
Sample : SARS-CoV infected 293T cells
Amount used : 10 μg
Blocking : BSA, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 10-Mar-2020
Score :
Application Tested : ICC/IF
Sample Species : SARS-CoV-2
Sample : BHK-21 transfected with full-length SARS-CoV-2 spike
Fixation : PFA, 0.5Hr
Primary Antibody : 1:2000, 4°C, 4Hr
Figure Note : Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Date : Anonymous submitted on 01-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV, SARS-CoV-2
Sample : SARS-CoV / SARS-CoV-2 infected Caco-2 cells
Amount used : 20 μg
Blocking : 5% milk in PBST, 20°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf