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SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR3022-RB]

Recombinant antibody

Cat. No. GTX01556

Reference 3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 CR3022-RB

Isotype

 IgG

Application

 ELISA, Neutralizing/Inhibition

Reactivity

 SARS Coronavirus, SARS Coronavirus 2
Package
100 μg ($399)
See all SARS-CoV-2 (COVID-19) Spike products
PRODUCT

Summary

This antibody binds to both SARS-CoV and SARS-CoV-2 with high affinity (PMID: 16796401 & 32065055). The initial characterization of the binding of this antibody was performed by ELISA and indicates potential for the development of diagnostic assays, as both virus-capture assays, or as controls in serological assays measuring immune responses to virus exposure. The original human IgG1 version of the antibody works synergistically in combination with another non-competing SARS antibody CR3014 and is a potential candidate for passive immune prophylaxis of SARS-CoV infection (ter Meulen et al., 2006). The original antibody (human IgG1) was also reported to bind the 2019-nCoV RBD (KD of 6.3 nM). This antibody may have potential as a therapeutic agent, alone or in combination with other neutralizing antibodies for treatment of 2019-nCoV infections (Tian et al., 2020).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Product Note

This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" confromation of the spike protein (Joyce et al. 2020)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Database

Research Area

REFERENCE

Application Reference

Lin HT et al. Int J Biol Macromol 2021; 193 (Pt B) : 1885-1897 Nanoparticular CpG-adjuvanted SARS-CoV-2 S1 protein elicits broadly neutralizing and Th1-biased immunoreactivity in mice.
Application : Neutralizing/Inhibition

Clone Reference

Tian X et al. Emerg Microbes Infect. 2020; 9 (1):382-385 Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody.
ter Meulen J et al. PLoS Med. 2006; 3 (7) Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants.
Application : Neutralizing/Inhibition
Submit a Reference

REVIEW

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SDS
PBS.pdf
Proclin.pdf
Package List Price ($)
$ 399