SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR3022] binds to both SARS-CoV and SARS-CoV-2 with high affinity (PMID: 16796401 & 32065055). The initial characterization of the binding of this antibody was performed by ELISA and indicates potential for the development of diagnostic assays, as both virus-capture assays, or as controls in serological assays measuring immune responses to virus exposure. The original human IgG1 version of the antibody works synergistically in combination with another non-competing SARS antibody CR3014 and is a potential candidate for passive immune prophylaxis of SARS-CoV infection (ter Meulen et al., 2006). The original antibody (human IgG1) was also reported to bind the SARS-CoV-2 RBD (KD of 6.3 nM). This antibody may have potential as a therapeutic agent, alone or in combination with other neutralizing antibodies for treatment of SARS-CoV-2 infections (Tian et al., 2020).
*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" confromation of the spike protein (Joyce et al. 2020)
0.02% ProClin 300
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ELISA analysis of SARS-CoV-2 Spike S1 protein, His tag (Insect Cells; grey line), SARS-CoV-2 Spike S2 protein, His tag (Insect Cells; yellow line) and SARS Coronavirus Spike S1 protein, His tag (HEK293 cells; blue line) at concentrations of 5 μg/ml using GTX01555 SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR3022]. A 3-fold serial dilution antibody from 41.6 ng/ml was performed. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.
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GTX01555 ELISA Image
ELISA analysis of SARS-CoV-2 Spike S1 protein, Sheep Fc tag (blue line) and SARS-CoV-2 Spike S2 protein, Sheep Fc tag (orange line) at concentrations of 5 μg/ml using GTX01555 SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [CR3022]. A 3-fold serial dilution primary antibody from 125 ng/ml was performed. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.