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SARS-CoV Spike overexpression 293T whole cell lysate

SARS-CoV Spike overexpression 293T whole cell lysate. GTX535668
SARS-CoV Spike overexpression 293T whole cell lysate. GTX535668

Cat No. GTX535668

Application

WB

Species

SARS Coronavirus
Package
200 μg ($299)
PRODUCT

Summary

Whole-cell lysate of 293T cells overexpressing SARS-CoV spike protein (full-length, no tag). The translated amino acid sequence is based on AAP13567.1. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293T lysate (GTX535673)

PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 μg/ml Leupeptin, 2 μg/ml Aprotinin, 1 μg/ml Pepstatin A, 0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

SARS-CoV Spike overexpression 293T whole cell lysate. GTX535668

GTX535668 WB Image

Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV Spike (SΔ3) antibody [7G12] (GTX632603) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 1509661

SARS-CoV Spike overexpression 293T whole cell lysate. GTX535668

GTX535668 WB Image

SARS-CoV spike 293T overexpression cell lysates (GTX535668) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV Spike (SΔ3) antibody [7G12] (GTX632603) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 1509661

REFERENCE

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REVIEW

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SDS
Proclin.pdf