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SART1 antibody [N2C1], Internal

Anti-SART1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103371
Anti-SART1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX103371
Anti-SART1 antibody [N2C1], Internal used in Western Blot (WB). GTX103371
Anti-SART1 antibody [N2C1], Internal used in Western Blot (WB). GTX103371

Cat No. GTX103371

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H929 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SART1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP , Ara1 , HAF , HOMS1 , SART1259 , SNRNP110 , Snu66

Cellular Localization

Nucleus

Background

This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SART1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103371

GTX103371 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using SART1(GTX103371) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SART1 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX103371

GTX103371 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SART1(GTX103371) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SART1 antibody [N2C1], Internal used in Western Blot (WB). GTX103371

GTX103371 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX103371 diluted at 1:5000

Anti-SART1 antibody [N2C1], Internal used in Western Blot (WB). GTX103371

GTX103371 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: NCI-H929
7.5% SDS PAGE
GTX103371 diluted at 1:5000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169