GeneTex
United States (US)

SART1 antibody

GTX64573_20180530_WB_18121411_711.jpg
GTX64573_20180530_IP_18121411_921.jpg

Cat No. GTX64573

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

90 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HOMS1 Antibody , SART1259 Antibody , SNRNP110 Antibody , SNU66 Antibody , ARA1 Antibody

Background

This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX64573_20180530_WB_18121411_711.jpg

GTX64573 WB Image

WB analysis of various samples using GTX64573 SART1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64573_20180530_IP_18121411_921.jpg

GTX64573 IP Image

IP analysis of Jurkat cell lysate using GTX64573 SART1 antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:1000

Package List Price ($)
$ 319