*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A431 , H1299 , HeLa , HepG2 , Raji
Knockdown/Knockout validation was supported by references (PMID:26990993)
Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.52 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SART3. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Spliceosome Associated Factor 3, U4/U6 Recycling Protein,Dsap1,P100,Rp11-13G14,Tip110,P110,P110(Nrb),Sart3
Cytoplasm , Nucleus speckle
The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq]
GTX107684 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
D: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX107684 diluted at 1:1000
GTX107684 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SART3 (GTX107684) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX107684 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SART3(GTX107684) antibody at 1:200 dilution.
GTX107684 IP Image
SART3 antibody [C3], C-term immunoprecipitates SART3 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SART3 protein by 4 μg SART3 antibody [C3], C-term (GTX107684)
5 % SDS-PAGE
The immunoprecipitated SART3 protein was detected by SART3 antibody [C3], C-term (GTX107684) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]