GeneTex
United States (US)

SART3 antibody [C3], C-term

GTX107684_WB_19011506_874.jpg
GTX107684_IHC_19011506_369.jpg
GTX107684_IFA_19011506_211.jpg
GTX107684_39757_IP_19011506_804.jpg

Cat No. GTX107684

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Raji

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SART3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Spliceosome Associated Factor 3, U4/U6 Recycling Protein,Dsap1,P100,Rp11-13G14,Tip110,P110,P110(Nrb),Sart3

Cellular Localization

Cytoplasm , Nucleus speckle

Background

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq]

Database

DATA IMAGES
GTX107684_WB_19011506_874.jpg

GTX107684 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX107684 diluted at 1:1000

GTX107684_IHC_19011506_369.jpg

GTX107684 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SART3 (GTX107684) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107684_IFA_19011506_211.jpg

GTX107684 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SART3(GTX107684) antibody at 1:200 dilution.

GTX107684_39757_IP_19011506_804.jpg

GTX107684 IP Image

SART3 antibody [C3], C-term immunoprecipitates SART3 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SART3 protein by 4 μg SART3 antibody [C3], C-term (GTX107684)
5 % SDS-PAGE
The immunoprecipitated SART3 protein was detected by SART3 antibody [C3], C-term (GTX107684) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169