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SART3 antibody [C3], C-term

KO/KD
Anti-SART3 antibody [C3], C-term used in Western Blot (WB). GTX107684
Anti-SART3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107684
Anti-SART3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107684
Anti-SART3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX107684

Cat. No. GTX107684

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SART3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SART3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951812

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spliceosome associated factor 3, U4/U6 recycling protein , DSAP1 , P100 , RP11-13G14 , TIP110 , p110 , p110(nrb)

Cellular Localization

Cytoplasm , Nucleus speckle

Background

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SART3 antibody [C3], C-term used in Western Blot (WB). GTX107684

GTX107684 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (GTX107684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SART3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107684

GTX107684 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SART3(GTX107684) antibody at 1:200 dilution.

Anti-SART3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107684

GTX107684 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SART3 (GTX107684) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SART3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX107684

GTX107684 IP Image

SART3 antibody [C3], C-term immunoprecipitates SART3 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SART3 protein by 4 μg SART3 antibody [C3], C-term (GTX107684)
5 % SDS-PAGE
The immunoprecipitated SART3 protein was detected by SART3 antibody [C3], C-term (GTX107684) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Rachid El Fatimy et al. Mol Cancer 2022; 21 (1) : 17 A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing.
Application : ICC/IF, IP
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF, IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169