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SASS6 antibody [N2C1], Internal

Anti-SASS6 antibody [N2C1], Internal used in Western Blot (WB). GTX118940
Anti-SASS6 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118940
Anti-SASS6 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX118940

Cat No. GTX118940

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SASS6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618103

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SAS-6 centriolar assembly protein , MCPH14 , SAS-6 , SAS6

Cellular Localization

Centrosome

Background

SAS6 is necessary for centrosome duplication and functions during procentriole formation; SAS6 functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle Strnad et al., (2007) [PubMed 17681132].[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SASS6 antibody [N2C1], Internal used in Western Blot (WB). GTX118940

GTX118940 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX118940 diluted at 1:10000

Anti-SASS6 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118940

GTX118940 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SASS6(GTX118940) antibody at 1:200 dilution.

Anti-SASS6 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX118940

GTX118940 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using SASS6(GTX118940) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for SASS6 antibody [N2C1], Internal (GTX118940). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SASS6 antibody [N2C1], Internal (GTX118940). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169