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SATB1 antibody [13D6]

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in Western Blot (WB). GTX83682
Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in Western Blot (WB). GTX83682
Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682
Anti-SATB1 antibody [13D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83682

Cat No. GTX83682

Host

Mouse

Clonality

Monoclonal

Clone Name

13D6

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SATB1 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SATB homeobox 1

Background

Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.

Database

DATA IMAGES

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in Western Blot (WB). GTX83682

GTX83682 WB Image

WB analysis of HEK293T cells transfected with SATB1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83682 SATB1 antibody [13D6].
Loading : 5 ug per lane

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of lung carcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of endometrium adenocarcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of bladder carcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in Western Blot (WB). GTX83682

GTX83682 WB Image

WB analysis of various cell lines using GTX83682 SATB1 antibody [13D6].
Loading : 35 ug per lane

Anti-SATB1 antibody [13D6] used in IHC (Paraffin sections) (IHC-P). GTX83682

GTX83682 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83682 SATB1 antibody [13D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SATB1 antibody [13D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83682

GTX83682 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SATB1 plasmid using GTX83682 SATB1 antibody [13D6].

REFERENCE

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Package List Price ($)
$ 409