SATB1 antibody [C2C3], C-term

SATB1 antibody immunoprecipitates SATB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of SATB1 protein by 2 μg of SATB1 antibody (GTX114738) 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody (GTX114738) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SATB1(GTX114738) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of SATB1 protein from 293T whole cell extract using 5 μg of SATB1 antibody [C2C3], C-term (GTX114738).
Western blot analysis was performed using SATB1 antibody [C2C3], C-term (GTX114738).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of SATB1 protein from 293T whole cell extract using 5 μg of SATB1 antibody [C2C3], C-term (GTX114738).
Western blot analysis was performed using SATB1 antibody [C2C3], C-term (GTX114738).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500.
Antigen Retrieval: Citra

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500.
Antigen Retrieval: Citrate

Cross-linked ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or anti-SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.