GeneTex
United States (US)

SATB1 antibody [C2C3], C-term

GTX114738_40499_IP_18121410_628.jpg
GTX114738_40499_ChIP_18121410_968.jpg
GTX114738_40499_IHC_18121410_765.jpg
GTX114738_40499_WB_2_18121410_172.jpg
GTX114738_40499_WB_18121410_180.jpg
GTX114738_40499_20170706_WB_M_thymus_18121410_269.jpg

Cat No. GTX114738

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

Jurkat , THP-1 , mouse thymus

Predict Reactivity

Rat, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SATB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Satb Homeobox 1,Satb1

Cellular Localization

Nucleus

Background

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX114738_40499_IP_18121410_628.jpg

GTX114738 IP Image

SATB1 antibody immunoprecipitates SATB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of SATB1 protein by 2 μg of SATB1 antibody (GTX114738) 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody (GTX114738) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX114738_40499_ChIP_18121410_968.jpg

GTX114738 ChIP assay Image

Cross-linked ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or anti-SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.
ChIP experiment and primer designs are based on Nucleic Acids Res. 2011 Jun;39(11):4640-52.

GTX114738_40499_IHC_18121410_765.jpg

GTX114738 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SATB1(GTX114738) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114738_40499_WB_2_18121410_172.jpg

GTX114738 WB Image

SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114738) diluted by 1:500

GTX114738_40499_WB_18121410_180.jpg

GTX114738 WB Image

SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114738) diluted by 1:500

GTX114738_40499_20170706_WB_M_thymus_18121410_269.jpg

GTX114738 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169