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SATB1 antibody [C2C3], C-term

Anti-SATB1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in ChIP assay (ChIP assay). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in Western Blot (WB). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114738
Anti-SATB1 antibody [C2C3], C-term used in Western Blot (WB). GTX114738

Cat. No. GTX114738

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SATB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

mouse thymus

Predict Reactivity

Rat, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SATB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167788

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SATB homeobox 1

Cellular Localization

Nucleus

Background

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SATB1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114738

GTX114738 IP Image

SATB1 antibody immunoprecipitates SATB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of SATB1 protein by 2 μg of SATB1 antibody (GTX114738) 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody (GTX114738) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SATB1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114738

GTX114738 ICC/IF Image

SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SATB1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114738

GTX114738 IP Image

Immunoprecipitation of SATB1 protein from 293T whole cell extract using 5 μg of SATB1 antibody [C2C3], C-term (GTX114738).
Western blot analysis was performed using SATB1 antibody [C2C3], C-term (GTX114738).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-SATB1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114738

GTX114738 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SATB1(GTX114738) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SATB1 antibody [C2C3], C-term used in ChIP assay (ChIP assay). GTX114738

GTX114738 ChIP assay Image

Cross-linked ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or anti-SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.
ChIP experiment and primer designs are based on Nucleic Acids Res. 2011 Jun;39(11):4640-52.

Anti-SATB1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114738

GTX114738 IHC-P Image

SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500.
Antigen Retrieval: Citrate

Anti-SATB1 antibody [C2C3], C-term used in Western Blot (WB). GTX114738

GTX114738 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SATB1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114738

GTX114738 IHC-P Image

SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500.
Antigen Retrieval: Citra

Anti-SATB1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114738

GTX114738 IP Image

Immunoprecipitation of SATB1 protein from 293T whole cell extract using 5 μg of SATB1 antibody [C2C3], C-term (GTX114738).
Western blot analysis was performed using SATB1 antibody [C2C3], C-term (GTX114738).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-SATB1 antibody [C2C3], C-term used in Western Blot (WB). GTX114738

GTX114738 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Li YC et al. Oral Dis 2016; (Epub) SATB1 promotes tumor metastasis and invasiveness in oral squamous cell carcinoma.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169