GeneTex
United States (US)

SATB1 antibody, Internal

GTX88290_2364_WB_18122102_442.jpg
GTX88290_977_IHC-P_18122102_751.jpg

Cat No. GTX88290

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Thymus shows select nuclear staining.

Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88290-PEP).

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ESDEENRQKTRPRT, from the internal region of the protein sequence according to NP_002962.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Satb Homeobox 1,Satb1

Cellular Localization

Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.

Background

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Database

DATA IMAGES
GTX88290_2364_WB_18122102_442.jpg

GTX88290 WB Image

WB analysis of Molt-4 cell lysate using GTX88290 SATB1 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88290_977_IHC-P_18122102_751.jpg

GTX88290 IHC-P Image

IHC-P analysis of human thymus using GTX88290 SATB1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

Package List Price ($)
$ 329