GeneTex
United States (US)

SATB1 antibody

GTX114737_40233_WB_18121410_762.jpg
GTX114737_40233_IHC_R_18121410_205.jpg
GTX114737_40233_IHC_18121410_597.jpg
GTX114737_40233_20170706_WB_M_thymus_18121410_745.jpg
GTX114737_40233_WB_2_18121410_113.jpg
GTX114737_40233_20170518_WB_M_R_18121410_917.jpg
GTX114737_40233_IHC_M_18121410_796.jpg

Cat No. GTX114737

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

Jurkat , MDA-MB-231 , THP-1 , mouse thymus , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SATB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SATB1 antibody, special AT-rich sequence-binding protein 1 antibody, DNA-binding protein SATB1 antibody, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA) antibody, SATB homeobox 1 antibody

Cellular Localization

Nucleus

Background

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

DATA IMAGES
GTX114737_40233_WB_18121410_762.jpg

GTX114737 WB Image

SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114737) diluted by 1:500

GTX114737_40233_IHC_R_18121410_205.jpg

GTX114737 IHC-P Image

SATB1 antibody detects SATB1 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
SATB1 antibody (GTX114737) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114737_40233_IHC_18121410_597.jpg

GTX114737 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using SATB1(GTX114737) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114737_40233_20170706_WB_M_thymus_18121410_745.jpg

GTX114737 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody (GTX114737) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114737_40233_WB_2_18121410_113.jpg

GTX114737 WB Image

SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114737) diluted by 1:500

GTX114737_40233_20170518_WB_M_R_18121410_917.jpg

GTX114737 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody (GTX114737) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114737_40233_IHC_M_18121410_796.jpg

GTX114737 IHC-P Image

SATB1 antibody detects SATB1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SATB1 antibody (GTX114737) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169