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SATB1 antibody

Anti-SATB1 antibody used in Western Blot (WB). GTX114737
Anti-SATB1 antibody used in Western Blot (WB). GTX114737
Anti-SATB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114737
Anti-SATB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114737
Anti-SATB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114737
Anti-SATB1 antibody used in Western Blot (WB). GTX114737
Anti-SATB1 antibody used in Western Blot (WB). GTX114737

Cat. No. GTX114737

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SATB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

THP-1 , Jurkat , mouse brain , rat brain , mouse thymus

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SATB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620618

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SATB homeobox 1

Cellular Localization

Nucleus

Background

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SATB1 antibody used in Western Blot (WB). GTX114737

GTX114737 WB Image

SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114737) diluted by 1:500

Anti-SATB1 antibody used in Western Blot (WB). GTX114737

GTX114737 WB Image

SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114737) diluted by 1:500

Anti-SATB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114737

GTX114737 IHC-P Image

SATB1 antibody detects SATB1 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
SATB1 antibody (GTX114737) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SATB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114737

GTX114737 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using SATB1(GTX114737) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SATB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114737

GTX114737 IHC-P Image

SATB1 antibody detects SATB1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SATB1 antibody (GTX114737) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SATB1 antibody used in Western Blot (WB). GTX114737

GTX114737 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody (GTX114737) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SATB1 antibody used in Western Blot (WB). GTX114737

GTX114737 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody (GTX114737) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169