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SATB2 antibody

Anti-SATB2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134135
Anti-SATB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134135
Anti-SATB2 antibody used in Western Blot (WB). GTX134135

Cat. No. GTX134135

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SATB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse SATB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887228

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

special AT-rich sequence binding protein 2 , BAP002 , mKIAA1034

Database

Research Area

DATA IMAGES

Anti-SATB2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134135

GTX134135 IHC-Fr Image

SATB2 antibody detects SATB2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: SATB2 stained by SATB2 antibody (GTX134135) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SATB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134135

GTX134135 ICC/IF Image

SATB2 antibody detects SATB2 protein at by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SATB2 stained by SATB2 antibody (GTX134135) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SATB2 antibody used in Western Blot (WB). GTX134135

GTX134135 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SATB2 antibody (GTX134135) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 14701874

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169