GeneTex
United States (US)

SATB2 antibody

GTX30894_858_IHC-P_20180221_18121410_192.jpg
GTX30894_72_IHC-P_20180221_18121410_631.jpg
GTX30894_2455_WB_20180221_18121410_212.jpg

Cat No. GTX30894

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 - 4 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , A20 Cell Lysate(GTX27180)

Specificity/Sensitivity

At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SATB2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human SATB2.The immunogen is located within amino acids 570 - 620 of SATB2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ21474, FLJ32076, KIAA1034, MGC119474, MGC119477

Background

Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development. At least two isoforms of SATB2 are known to exist.

Database

DATA IMAGES
GTX30894_858_IHC-P_20180221_18121410_192.jpg

GTX30894 IHC-P Image

IHC-P analysis of human brain tissue using GTX30894 SATB2 antibody.
Working concentration : 5 μg/ml

GTX30894_72_IHC-P_20180221_18121410_631.jpg

GTX30894 IHC-P Image

IHC-P analysis of human brain tissue using GTX30894 SATB2 antibody.
Working concentration : 50 μg/ml

GTX30894_2455_WB_20180221_18121410_212.jpg

GTX30894 WB Image

WB analysis of A-20 cell lysate using GTX30894 SATB2 antibody.
Working concentration : (A) 2 and (B) 4 μg/ml

Package List Price ($)
$ 319