GeneTex
United States (US)

SCAMP3 antibody [N1N3]

GTX102216_39728_IHC_19011506_380.jpg
GTX102216_WB_19011506_365.jpg
GTX102216_39728_IFA_19011506_948.jpg

Cat No. GTX102216

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

H1299 , Molt-4

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCAMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C1orf3 antibody, SCAMP3 antibody, propin 1 antibody, secretory carrier-associated membrane protein 3 antibody, secretory carrier membrane protein 3 antibody

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene product belongs to the SCAMP family of proteins which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that the SCAMPs may function at the same site during vesicular transport rather than in separate pathways. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX102216_39728_IHC_19011506_380.jpg

GTX102216 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adrenal gland, using SCAMP3(GTX102216) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102216_WB_19011506_365.jpg

GTX102216 WB Image

Sample(30 µg of whole cell lysate)
A:MOLT4(GTX27912)
12% SDS PAGE
GTX102216 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102216_39728_IFA_19011506_948.jpg

GTX102216 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SCAMP3(GTX102216) antibody at 1:1000 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169