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SCAP antibody [HL2796]

Recombinant antibody
Anti-SCAP antibody [HL2796] used in Western Blot (WB). GTX639684
Anti-SCAP antibody [HL2796] used in Western Blot (WB). GTX639684
Anti-SCAP antibody [HL2796] used in IHC (Paraffin sections) (IHC-P). GTX639684
Anti-SCAP antibody [HL2796] used in IHC (Paraffin sections) (IHC-P). GTX639684

Cat. No. GTX639684

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2796

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SCAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human SCAP.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SREBF chaperone

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane

Background

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-SCAP antibody [HL2796] used in Western Blot (WB). GTX639684

GTX639684 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [HL2796] (GTX639684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SCAP antibody [HL2796] used in Western Blot (WB). GTX639684

GTX639684 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [HL2796] (GTX639684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SCAP antibody [HL2796] used in IHC (Paraffin sections) (IHC-P). GTX639684

GTX639684 IHC-P Image

SCAP antibody [HL2796] detects SCAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
SCAP stained by SCAP antibody [HL2796] (GTX639684) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SCAP antibody [HL2796] used in IHC (Paraffin sections) (IHC-P). GTX639684

GTX639684 IHC-P Image

SCAP antibody [HL2796] detects SCAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SCAP stained by SCAP antibody [HL2796] (GTX639684) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Available soon.
Custom Conjugations