APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89599-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GLVPKAYIMEMYDI, from the C Terminus of the protein sequence according to NP_003921.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
src kinase associated phosphoprotein 2 , PRAP , RA70 , SAPS , SCAP2 , SKAP-HOM , SKAP55R
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene shares homology with Src kinase-associated phosphoprotein 1, and is a substrate of Src family kinases. It is an adaptor protein that is thought to play an essential role in the Src signaling pathway, and in regulating proper activation of the immune system. This protein contains an amino terminal coiled-coil domain for self-dimerization, a plecskstrin homology (PH) domain required for interactions with lipids at the membrane, and a Src homology (SH3) domain at the carboxy terminus. Some reports indicate that this protein inhibits actin polymerization through interactions with actin assembly factors, and might negatively regulate the invasiveness of tumors by modulating actin assembly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
Database
Research Area
DATA IMAGES
|
GTX89599 WB Image
WB analysis of Jurkat lysate using GTX89599 SCAP2 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX89599 WB Image
WB analysis of HEK293 overexpressing SCAP2 (mock transfection in first lane) using GTX89599 SCAP2 antibody, C-term.
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for SCAP2 antibody, C-term (GTX89599). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SCAP2 antibody, C-term (GTX89599). Be the first to share your experience with this product.
