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SCARA3 antibody [N2C2], Internal

Anti-SCARA3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100595
Anti-SCARA3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100595
Anti-SCARA3 antibody [N2C2], Internal used in Western Blot (WB). GTX100595

Cat No. GTX100595

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCARA3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

scavenger receptor class A member 3 , APC7 , CSR , CSR1 , MSLR1 , MSRL1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein , Golgi apparatus membrane; Single-pass type II membrane protein

Background

This gene encodes a macrophage scavenger receptor-like protein. This protein has been shown to deplete reactive oxygen species, and thus play an important role in protecting cells from oxidative stress. The expression of this gene is induced by oxidative stress. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

DATA IMAGES

Anti-SCARA3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100595

GTX100595 ICC/IF Image

SCARA3 antibody [N2C2], Internal detects SCARA3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min.
Green: SCARA3 protein stained by SCARA3 antibody [N2C2], Internal (GTX100595) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-SCARA3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100595

GTX100595 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using SCARA3(GTX100595) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SCARA3 antibody [N2C2], Internal used in Western Blot (WB). GTX100595

GTX100595 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX100595 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169