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SCARA3 antibody [N2C2], Internal

Anti-SCARA3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100595
Anti-SCARA3 antibody [N2C2], Internal used in Western Blot (WB). GTX100595
Anti-SCARA3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100595

Cat. No. GTX100595

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SCARA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human SCARA3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951818

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

scavenger receptor class A member 3 , APC7 , CSR , CSR1 , MSLR1 , MSRL1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein , Golgi apparatus membrane; Single-pass type II membrane protein

Background

This gene encodes a macrophage scavenger receptor-like protein. This protein has been shown to deplete reactive oxygen species, and thus play an important role in protecting cells from oxidative stress. The expression of this gene is induced by oxidative stress. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SCARA3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100595

GTX100595 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using SCARA3(GTX100595) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SCARA3 antibody [N2C2], Internal used in Western Blot (WB). GTX100595

GTX100595 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX100595 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SCARA3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100595

GTX100595 ICC/IF Image

SCARA3 antibody [N2C2], Internal detects SCARA3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min.
Green: SCARA3 protein stained by SCARA3 antibody [N2C2], Internal (GTX100595) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

Application Reference

Salhi A et al. Am J Pathol 2020; 190 (5) : 1108-1117 Oxidative Phosphorylation Promotes Primary Melanoma Invasion.
Application : IHC-P
Reactivity : Human
Atilano SR et al. J Ophthalmic Vis Res 2019; 14 (1) : 62-70 Corneal Oxidative Damage in Keratoconus Cells due to Decreased Oxidant Elimination from Modified Expression Levels of SOD Enzymes, PRDX6, SCARA3, CPSF3, and FOXM1.
Application : IHC
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169