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SCARA5 antibody

Anti-SCARA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85270
Anti-SCARA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85270
Anti-SCARA5 antibody used in Western Blot (WB). GTX85270

Cat No. GTX85270

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Hep G2 Cell Slide(GTX25530) , Human Spleen Tissue Lysate(GTX27921)

Product Note

At least four isoforms of SCARA5 are known to exist; this antibody will detect all four isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SCARA5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SCARA5.The immunogen is located within amino acids 350 - 400 of SCARA5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10723521

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

scavenger receptor class A member 5 , NET33 , Tesr

Cellular Localization

Cell membrane

Background

Scavenger receptor class A member 5 (SCARA5), also known as TESR, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARA5 is a cell-surface receptor that contains that collagen-like domain and N-terminal scavenger receptor cysteine rich domain typical of the class A scavenger receptor subfamily. Unlike other class A receptors, SCARA5 does not endocytose acetylated or oxidized low density lipoprotein. Instead, SCARA5 binds serum ferritin and mediates its endocytosis from the cell surface, indicating SCARA5 may play a role in non-transferrin iron delivery. At least four isoforms of SCARB1 are known to exist.

Database

Research Area

DATA IMAGES

Anti-SCARA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85270

GTX85270 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX85270 SCARA5 antibody.
Working concentration : 20 μg/ml

Anti-SCARA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85270

GTX85270 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX85270 SCARA5 antibody.
Working concentration : 2.5 μg/ml

Anti-SCARA5 antibody used in Western Blot (WB). GTX85270

GTX85270 WB Image

WB analysis of human liver tissue lysate using GTX85270 SCARA5 antibody.
Working concentration : 1 μg/ml

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf