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SCD antibody

Anti-SCD antibody used in Western Blot (WB). GTX04643
Anti-SCD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04643
Anti-SCD antibody used in IHC (Paraffin sections) (IHC-P). GTX04643
Anti-SCD antibody used in IHC (Paraffin sections) (IHC-P). GTX04643

Cat. No. GTX04643

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SCD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SCD(Accession O00767), corresponding to amino acid residues V27-I77.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stearoyl-CoA desaturase , FADS5 , MSTP008 , SCD1 , SCDOS , hSCD1

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-SCD antibody used in Western Blot (WB). GTX04643

GTX04643 WB Image

WB analysis of HeLa whole cell lysate using GTX04643 SCD antibody. The lane on the left was treated with blocking peptide.

Anti-SCD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04643

GTX04643 ICC/IF Image

ICC/IF analysis PFA-fixed HeLa cells using GTX04643 SCD antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-SCD antibody used in IHC (Paraffin sections) (IHC-P). GTX04643

GTX04643 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04643 SCD antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-SCD antibody used in IHC (Paraffin sections) (IHC-P). GTX04643

GTX04643 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX04643 SCD antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399