GeneTex
United States (US)

SCGN antibody [N1C2]

GTX115442_40401_IHC_18121410_922.jpg
GTX115442_40401_IFA_18121410_159.jpg
GTX115442_40401_WB_18121410_560.jpg

Cat No. GTX115442

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.79mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CALBL antibody, DJ501N12.8 antibody, SECRET antibody, SEGN antibody, setagin antibody, SCGN antibody, secretagogin antibody, "secretagogin, EF-hand calcium binding protein antibody"

Cellular Localization

Cytoplasm , Secreted (By similarity) , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity)

Background

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]
Show More +

Database

Research Area

DATA IMAGES
GTX115442_40401_IHC_18121410_922.jpg

GTX115442 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SCGN(GTX115442) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115442_40401_IFA_18121410_159.jpg

GTX115442 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SCGN(GTX115442) antibody at 1:200 dilution.

GTX115442_40401_WB_18121410_560.jpg

GTX115442 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX115442 diluted at 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf