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SCGN antibody [N1C2]

Anti-SCGN antibody [N1C2] used in Western Blot (WB). GTX115442
Anti-SCGN antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115442
Anti-SCGN antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115442

Cat. No. GTX115442

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SCGN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10728906

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secretagogin, EF-hand calcium binding protein , CALBL , DJ501N12.8 , SECRET , SEGN , setagin

Cellular Localization

Cytoplasm , Secreted , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side

Background

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SCGN antibody [N1C2] used in Western Blot (WB). GTX115442

GTX115442 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX115442 diluted at 1:5000

Anti-SCGN antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115442

GTX115442 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SCGN(GTX115442) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SCGN antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115442

GTX115442 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SCGN(GTX115442) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169