APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , mouse brain , rat brain
Predict Reactivity
Zebrafish, Bovine, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10719884
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
secretagogin, EF-hand calcium binding protein , CALBL , DJ501N12.8 , SECRET , SEGN , setagin
Cellular Localization
Cytoplasm , Secreted , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side
Background
The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115443 IHC-Fr Image
SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green:SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX115443 IHC-Fr Image
SCGN antibody [N2C3] detects SCGN Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX115443 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCGN(GTX115443) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115443 ICC/IF Image
SCGN antibody [N2C3] detects SCGN protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX115443 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SCGN antibody [N2C3] (GTX115443) diluted at 1:500.
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GTX115443 IHC-Fr Image
SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX115443 WB Image
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE GTX115443 diluted at 1:1000
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REFERENCE
There are currently no references for SCGN antibody [N2C3] (GTX115443). Be the first to share your publications with this product.
REVIEW
SCGN antibody [N2C3]
Cat. No. GTX115443
Rating
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( Average 5 based on 1 users reviews)
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Application
IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 15-Dec-2020
Application Tested :
IHC-Fr
Sample Species :
Ms
Sample :
Olfactory bulb, free floating section
Blocking :
1% BSA/PBS, 25°C, 0.5Hr
Fixation :
4% PFA/PBS
Permeabilization /Antigen retrieval :
0.3% Triton X-100
Primary Antibody :
1:500, 4°C, 16Hr