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SCGN antibody [N2C3]

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Anti-SCGN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115443
Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115443

Cat. No. GTX115443

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719884

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secretagogin, EF-hand calcium binding protein , CALBL , DJ501N12.8 , SECRET , SEGN , setagin

Cellular Localization

Cytoplasm , Secreted , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side

Background

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green:SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443

GTX115443 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX115443 diluted at 1:1000

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-SCGN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115443

GTX115443 ICC/IF Image

SCGN antibody [N2C3] detects SCGN protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443

GTX115443 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SCGN antibody [N2C3] (GTX115443) diluted at 1:500.

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SCGN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115443

GTX115443 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCGN(GTX115443) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for SCGN antibody [N2C3] (GTX115443). Be the first to share your publications with this product.

REVIEW

SCGN antibody [N2C3] Cat. No. GTX115443

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Dec-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Olfactory bulb, free floating section
Blocking : 1% BSA/PBS, 25°C, 0.5Hr
Fixation : 4% PFA/PBS
Permeabilization /Antigen retrieval : 0.3% Triton X-100
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169