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SCGN antibody [N2C3]

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115443
Anti-SCGN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115443
Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443

Cat No. GTX115443

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa , HCT116 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719884

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

secretagogin, EF-hand calcium binding protein , CALBL , DJ501N12.8 , SECRET , SEGN , setagin

Cellular Localization

Cytoplasm , Secreted , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side

Background

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green:SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-SCGN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115443

GTX115443 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCGN(GTX115443) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SCGN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115443

GTX115443 ICC/IF Image

SCGN antibody [N2C3] detects SCGN protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443

GTX115443 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SCGN antibody [N2C3] (GTX115443) diluted at 1:500.

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443

GTX115443 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX115443 diluted at 1:1000

REFERENCE

There are currently no references for SCGN antibody [N2C3] (GTX115443). Be the first to share your publications with this product.

REVIEW

SCGN antibody [N2C3] Cat No. GTX115443

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Dec-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Olfactory bulb, free floating section
Blocking : 1% BSA/PBS, 25°C, 0.5Hr
Fixation : 4% PFA/PBS
Permeabilization /Antigen retrieval : 0.3% Triton X-100
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169