GeneTex
United States (US)

SCGN antibody [N2C3]

GTX115443_40415_20170214_IHC-Fr_18121410_830.jpg
GTX115443_40415_20170831_IHC-Fr_M_18121410_921.jpg
GTX115443_40415_IHC_18121410_148.jpg
GTX115443_40415_20171115_ICC_IF_R_18121410_712.jpg
GTX115443_40415_20161027_WB_M_R_18121410_839.jpg
GTX115443_40415_20161012_IHC-Fr_R_18121410_223.jpg
GTX115443_40415_WB_18121410_923.jpg

Cat No. GTX115443

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa , HCT116 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Secretagogin, Ef-Hand Calcium Binding Protein,Calbl,Dj501N12.8,Secret,Segn,Setagin,Scgn

Cellular Localization

Cytoplasm , Secreted , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side

Background

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115443_40415_20170214_IHC-Fr_18121410_830.jpg

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green:SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115443_40415_20170831_IHC-Fr_M_18121410_921.jpg

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX115443_40415_IHC_18121410_148.jpg

GTX115443 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCGN(GTX115443) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115443_40415_20171115_ICC_IF_R_18121410_712.jpg

GTX115443 ICC/IF Image

SCGN antibody [N2C3] detects SCGN protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115443_40415_20161027_WB_M_R_18121410_839.jpg

GTX115443 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SCGN antibody [N2C3] (GTX115443) diluted at 1:500.

GTX115443_40415_20161012_IHC-Fr_R_18121410_223.jpg

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115443_40415_WB_18121410_923.jpg

GTX115443 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX115443 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169