APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , mouse brain , rat brain
Predict Reactivity
Zebrafish, Bovine, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10719884
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
secretagogin, EF-hand calcium binding protein , CALBL , DJ501N12.8 , SECRET , SEGN , setagin
Cellular Localization
Cytoplasm , Secreted , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side
Background
The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115443 IHC-Fr Image
SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green:SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX115443 ICC/IF Image
SCGN antibody [N2C3] detects SCGN protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX115443 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SCGN antibody [N2C3] (GTX115443) diluted at 1:500.
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GTX115443 IHC-Fr Image
SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX115443 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCGN(GTX115443) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115443 WB Image
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE GTX115443 diluted at 1:1000
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GTX115443 IHC-Fr Image
SCGN antibody [N2C3] detects SCGN Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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REFERENCE
There are currently no references for SCGN antibody [N2C3] (GTX115443). Be the first to share your publications with this product.
REVIEW
SCGN antibody [N2C3]
Cat. No. GTX115443
Rating
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( Average 5 based on 1 users reviews)
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Application
IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 15-Dec-2020
Application Tested :
IHC-Fr
Sample Species :
Ms
Sample :
Olfactory bulb, free floating section
Blocking :
1% BSA/PBS, 25°C, 0.5Hr
Fixation :
4% PFA/PBS
Permeabilization /Antigen retrieval :
0.3% Triton X-100
Primary Antibody :
1:500, 4°C, 16Hr