GeneTex
United States (US)

SCMH1 antibody [N1N3]

GTX106946_IFA_19011506_614.jpg
GTX106946_IHC_19011506_188.jpg

Cat No. GTX106946

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , H1299 , HepG2 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCMH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Scml3 antibody, SCMH1 antibody, polycomb protein SCMH1 antibody, sex comb on midleg homolog 1 (Drosophila) antibody

Cellular Localization

Nucleus

Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Database

DATA IMAGES
GTX106946_IFA_19011506_614.jpg

GTX106946 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SCMH1(GTX106946) antibody at 1:200 dilution.

GTX106946_IHC_19011506_188.jpg

GTX106946 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SCMH1(GTX106946) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169