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SCMH1 antibody [N1N3]

Anti-SCMH1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106946
Anti-SCMH1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX106946

Cat. No. GTX106946

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SCMH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCMH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951820

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Scm polycomb group protein homolog 1 , Scml3

Cellular Localization

Nucleus

Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Database

Research Area

DATA IMAGES

Anti-SCMH1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106946

GTX106946 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SCMH1(GTX106946) antibody at 1:200 dilution.

Anti-SCMH1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX106946

GTX106946 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SCMH1(GTX106946) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169