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SCO1 antibody

Anti-SCO1 antibody used in Western Blot (WB). GTX85056
Anti-SCO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85056
Anti-SCO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85056

Cat No. GTX85056

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SCO1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human SCO1.The immunogen is located within amino acids 100 - 150 of SCO1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SCO cytochrome c oxidase assembly protein 1 , SCOD1

Cellular Localization

Mitochondrion,Mitochondrion inner membrane

Background

Synthesis of cytochrome c oxidase 1 was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. Like their yeast homologs, the function of both SCO1 and SCO2 are dependent on copper ion binding. Mutations in either gene can lead to cytochrome c oxidase respiratory chain defects, with a missense mutation in human SCO1 (P174L) associated with a fatal neonatal hepatopathy when the second allele is also non-functional, suggesting the pathology is due to loss of function. It has been suggested that this mutation alters the SCO1 affinity for the copper (I) ion, thus impairing the efficiency of copper transfer to the cytochrome c oxidase. At least two isoforms of SCO1 are known to exist and both are recognized by the SCO1 antibody. This SCO1 antibody has no cross-reactivity to SCO2.

Database

Research Area

DATA IMAGES

Anti-SCO1 antibody used in Western Blot (WB). GTX85056

GTX85056 WB Image

WB analysis of human brain tissue lysate using GTX85056 SCO1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-SCO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85056

GTX85056 IHC-P Image

IHC-P analysis of human brain tissue using GTX85056 SCO1 antibody.
Working concentration : 20 μg/ml

Anti-SCO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85056

GTX85056 IHC-P Image

IHC-P analysis of human brain tissue using GTX85056 SCO1 antibody.
Working concentration : 2.5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf