GeneTex
United States (US)

SCP2 antibody, Internal

GTX88705_865_IHC-P_18122102_913.jpg

Cat No. GTX88705

Host Goat
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 3-6μg/ml

Note :

Human Liver shows speckled cytoplasm staining in hepatocytes.
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88705-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2; NP_001007099.1; NP_001180529.1; NP_001180528.1; NP_001180546.1 respectively),

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-KNHKHSVNNPYSQ, from the internal region of the protein sequence according to NP_001007099.1; NP_002970.2; NP_001180528.1; NP_001180529.1; NP_001180546.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SCP CHI Antibody , NSL TP Antibody , NLTP Antibody , SCPX Antibody , SCP X Antibody

Cellular Localization

Cytoplasmic in the liver and also associated with mitochondria especially in steroidogenic tissues. Interaction with PEX5 is essential for peroxisomal import.

Background

This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.[provided by RefSeq, Aug 2010]
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Database

Research Area

DATA IMAGES
GTX88705_865_IHC-P_18122102_913.jpg

GTX88705 IHC-P Image

IHC-P analysis of human liver- using GTX88705 SCP2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml