APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HCT116
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SCP2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10722024
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
sterol carrier protein 2 , NLTP , NSL-TP , SCP-2 , SCP-CHI , SCP-X , SCPX
Cellular Localization
Cytoplasm , Mitochondrion , Isoform SCPx: Peroxisome
Background
This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. The full-length nature of all transcript variants has not been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-SCP2 antibody [N1C1] used in Western Blot (WB). GTX107339](/templates/default/js/lightbox/images/loading.gif "Anti-SCP2 antibody [N1C1] used in Western Blot (WB). GTX107339")
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GTX107339 WB Image
Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX107339 diluted at 1:1000
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GTX107339 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using SCP2(GTX107339) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for SCP2 antibody [N1C1] (GTX107339). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SCP2 antibody [N1C1] (GTX107339). Be the first to share your experience with this product.
![Anti-SCP2 antibody [N1C1] used in Western Blot (WB). GTX107339](/upload/website/prouct_img/small/GTX107339/GTX107339_40415_WB_19011506_927.jpg "Anti-SCP2 antibody [N1C1] used in Western Blot (WB). GTX107339")
![Anti-SCP2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107339](/upload/website/prouct_img/small/GTX107339/GTX107339_40415_IHC_19011506_936.jpg "Anti-SCP2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107339")
![Anti-SCP2 antibody [N1C1] used in Western Blot (WB). GTX107339](/upload/website/prouct_img/normal/GTX107339/GTX107339_40415_WB_19011506_927.jpg "Anti-SCP2 antibody [N1C1] used in Western Blot (WB). GTX107339")
![Anti-SCP2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107339](/upload/website/prouct_img/normal/GTX107339/GTX107339_40415_IHC_19011506_936.jpg "Anti-SCP2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX107339")