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SCRIB antibody

Anti-SCRIB antibody used in IHC (Paraffin sections) (IHC-P). GTX107692
Anti-SCRIB antibody used in Western Blot (WB). GTX107692
Anti-SCRIB antibody used in Western Blot (WB). GTX107692

Cat No. GTX107692

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

175 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3 , JC

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SCRIB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

scribble planar cell polarity protein , CRIB1 , SCRB1 , SCRIB1 , Vartul

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization (summary by Nola et al., 2008 [PubMed 18716323]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SCRIB antibody used in IHC (Paraffin sections) (IHC-P). GTX107692

GTX107692 IHC-P Image

SCRIB antibody detects SCRIB protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
SCRIB antibody (GTX107692) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SCRIB antibody used in Western Blot (WB). GTX107692

GTX107692 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
5% SDS PAGE
GTX107692 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SCRIB antibody used in Western Blot (WB). GTX107692

GTX107692 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
5% SDS PAGE
GTX107692 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for SCRIB antibody (GTX107692). Be the first to share your publications with this product.

REVIEW

SCRIB antibody Cat No. GTX107692

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 18-Dec-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human iPS cells
Amount used : 10 μg
Blocking : 5% skim milk in 0.05% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169