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SCUBE1 antibody

Anti-SCUBE1 antibody used in Western Blot (WB). GTX85174
Anti-SCUBE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85174
Anti-SCUBE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85174

Cat. No. GTX85174

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all SCUBE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

Daudi Cell Lysate(GTX23951)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SCUBE1 antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1.The immunogen is located within amino acids 460 - 510 of SCUBE1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal peptide, CUB domain and EGF like domain containing 1

Cellular Localization

Secreted,Cell membrane

Background

SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation of adhesions in a Ca2+-dependent fashion. In contrast, the C-terminal cysteine-rich motifs and CUB domain can directly bind and antagonize bone morphogenic protein activity; genetic ablation of this region resulted in brain malformation in mice embryos, suggesting SCUBE1 plays a role in brain morphogenesis. SCUBE1 has also been implicated in early craniofacial development in mice.

Database

Research Area

DATA IMAGES

Anti-SCUBE1 antibody used in Western Blot (WB). GTX85174

GTX85174 WB Image

WB analysis of Daudi cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85174 SCUBE1 antibody.
Working concentration : 1 μg/ml

Anti-SCUBE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85174

GTX85174 ICC/IF Image

ICC/IF analysis of Daudi cells using GTX85174 SCUBE1 antibody.
Working concentration : 20 μg/ml

Anti-SCUBE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85174

GTX85174 ICC/IF Image

ICC/IF analysis of Daudi cells using GTX85174 SCUBE1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399