APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SCYL1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951830
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SCY1 like pseudokinase 1 , GKLP , HT019 , NKTL , NTKL , P105 , SCAR21 , TAPK , TEIF , TRAP
Cellular Localization
Centrosome , Endoplasmic reticulum-Golgi intermediate compartment , Golgi apparatus , cis-Golgi network , Isoform 1: Cytoplasm , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 6: Nucleus
Background
This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
DATA IMAGES
![Anti-SCYL1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111355](/templates/default/js/lightbox/images/loading.gif "Anti-SCYL1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111355")
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GTX111355 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SCYL1(GTX111355) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
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GTX111355 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX111355 diluted at 1:1000
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REFERENCE
There are currently no references for SCYL1 antibody [N3C2], Internal (GTX111355). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SCYL1 antibody [N3C2], Internal (GTX111355). Be the first to share your experience with this product.
![Anti-SCYL1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111355](/upload/website/prouct_img/small/GTX111355/GTX111355_40058_IFA_w_23060500_850.webp "Anti-SCYL1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111355")
![Anti-SCYL1 antibody [N3C2], Internal used in Western Blot (WB). GTX111355](/upload/website/prouct_img/small/GTX111355/GTX111355_WB_w_23060500_840.webp "Anti-SCYL1 antibody [N3C2], Internal used in Western Blot (WB). GTX111355")
![Anti-SCYL1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111355](/upload/website/prouct_img/normal/GTX111355/GTX111355_40058_IFA_w_23060500_850.webp "Anti-SCYL1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111355")
![Anti-SCYL1 antibody [N3C2], Internal used in Western Blot (WB). GTX111355](/upload/website/prouct_img/normal/GTX111355/GTX111355_WB_w_23060500_840.webp "Anti-SCYL1 antibody [N3C2], Internal used in Western Blot (WB). GTX111355")