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SCYL2 antibody

KO/KD
Anti-SCYL2 antibody used in Western Blot (WB). GTX00732
Anti-SCYL2 antibody used in Western Blot (WB). GTX00732
Anti-SCYL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00732

Cat. No. GTX00732

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat, Hamster
Package
50 μg ($399)
See all SCYL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:200-1:1000
IP 1:200-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.4), 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human SCYL2 protein (amino acids 528-929) fused with a 6 * His tag

Purification

Purified IgG by Salting-out and ion-exchange chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SCY1 like pseudokinase 2 , CVAK104

Cellular Localization

Endosome membrane

Background

The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-SCYL2 antibody used in Western Blot (WB). GTX00732

GTX00732 WB Image

KO/KD

WB analysis of 293 cell lysate using GTX00732 SCYL2 antibody.
Samples :
Lane 1 : 293 cells transfected with control siRNA
Lane 2 : 293 cells transfected with si-SCYL2

Anti-SCYL2 antibody used in Western Blot (WB). GTX00732

GTX00732 WB Image

WB analysis of various samples using GTX00732 SCYL2 antibody.
Dilution : 1:1000
Loading : 20μg

Anti-SCYL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00732

GTX00732 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX00732 SCYL2 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399