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SCYL2 antibody

Anti-SCYL2 antibody used in Western Blot (WB). GTX66174
Anti-SCYL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66174
Anti-SCYL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66174

Cat. No. GTX66174

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SCYL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-300 of human SCYL2 (NP_060458.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SCY1 like pseudokinase 2 , CVAK104

Cellular Localization

Endosome membrane

Background

The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-SCYL2 antibody used in Western Blot (WB). GTX66174

GTX66174 WB Image

WB analysis of various sample lysates using GTX66174 SCYL2 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-SCYL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66174

GTX66174 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66174 SCYL2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SCYL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66174

GTX66174 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66174 SCYL2 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399