GeneTex
United States (US)

SCYL3 antibody [N1C3]

GTX103501_IFA_19011506_919.jpg
GTX103501_WB_19011506_881.jpg
GTX103501_IHC_19011506_738.jpg

Cat No. GTX103501

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SCYL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Scy1 Like Pseudokinase 3,Pace-1,Pace1,Scyl3

Cellular Localization

Cytoplasm , Golgi apparatus , Cell projection , lamellipodium

Background

May play a role in regulating cell adhesion/migration complexes in migrating cells.

Database

Research Area

DATA IMAGES
GTX103501_IFA_19011506_919.jpg

GTX103501 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SCY1 like 3(GTX103501) antibody at 1:200 dilution.

GTX103501_WB_19011506_881.jpg

GTX103501 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX103501 diluted at 1:1000

GTX103501_IHC_19011506_738.jpg

GTX103501 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using SCY1 like 3(GTX103501) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169