APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.5μg/ml |
| 0.5-1μg/ml |
| 0.1-0.5μg/ml |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄
Preservative
0.05mg Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
E. coli-derived human CXCL12 recombinant protein (Position: V24-M93).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
C-X-C motif chemokine ligand 12 , IRH , PBSF , SCYB12 , SDF1 , TLSF , TPAR1
Cellular Localization
Secreted
Background
This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Database
Research Area
DATA IMAGES
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GTX02876 WB Image
WB analysis of various samples using GTX02876 SDF1 antibody.
Lane 1 : human placenta tissue lysate
Lane 2 : A431 whole cell lysate
Dilution : 0.5 μg/ml
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GTX02876 IHC-P Image
IHC-P analysis of rat spleen tissue using GTX02876 SDF1 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml
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GTX02876 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX02876 SDF1 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml
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GTX02876 IHC-P Image
IHC-P analysis of human endometrial carcinoma tissue using GTX02876 SDF1 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml
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GTX02876 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX02876 SDF1 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml
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REFERENCE
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REVIEW
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