APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T
Predict Reactivity
Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888322
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF
Cellular Localization
Mitochondrion inner membrane
Background
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX632636 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.
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GTX632636 WB Image
HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX632636 ICC/IF Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.
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GTX632636 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.
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GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632636 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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|
GTX632636 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.
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REFERENCE
There are currently no references for SDHA antibody [GT20710] (GTX632636). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SDHA antibody [GT20710] (GTX632636). Be the first to share your experience with this product.