GeneTex
United States (US)

SDHA antibody [GT20710]

KO/KD
GTX632636_42289_20160825_WB_shRNA_watermark_18121411_707.jpg
GTX632636_42289_20151119_WB_18121411_266.jpg
GTX632636_42702_20170315_IFA_18121411_937.jpg
GTX632636_42702_20181207_IHC-P_M_19011519_821.jpg
GTX632636_42702_20181207_IHC-P_M_1_19011519_143.jpg
GTX632636_42702_20181207_IHC-P_R_19011519_657.jpg

Cat No. GTX632636

Host Mouse
Clonality Monoclonal
Clone Name GT20710
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Zebrafish, Drosophila, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.52mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PGL5 Antibody , SDH2 Antibody , succinate dehydrogenase complex, subunit A, flavoprotein (Fp) Antibody , SDHF Antibody , CMD1GG Antibody , SDH1 Antibody , FP Antibody

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX632636_42289_20160825_WB_shRNA_watermark_18121411_707.jpg

GTX632636 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.

GTX632636_42289_20151119_WB_18121411_266.jpg

GTX632636 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.

GTX632636_42702_20170315_IFA_18121411_937.jpg

GTX632636 ICC/IF Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.

GTX632636_42702_20181207_IHC-P_M_19011519_821.jpg

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632636_42702_20181207_IHC-P_M_1_19011519_143.jpg

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632636_42702_20181207_IHC-P_R_19011519_657.jpg

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169