GeneTex

  • Country / Location Selection

United States (US)

SDHA antibody [HL1067]

Recombinant antibody KO/KD
Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098
Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098
Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098
Anti-SDHA antibody [HL1067] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636098
Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098
Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098
Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098

Cat. No. GTX636098

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1067

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Zebrafish, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all SDHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SDHA. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909944

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098

GTX636098 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098

GTX636098 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098

GTX636098 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1067] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636098

GTX636098 ICC/IF Image

SDHA antibody [HL1067] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA stained by SDHA antibody [HL1067] (GTX636098) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098

GTX636098 WB Image

Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098

GTX636098 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1067] used in Western Blot (WB). GTX636098

GTX636098 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1067] (GTX636098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for SDHA antibody [HL1067] (GTX636098). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SDHA antibody [HL1067] (GTX636098). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations