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SDHA antibody [HL1117]

Recombinant antibody KO/KD Orthogonal
Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335
Anti-SDHA antibody [HL1117] used in IHC (Paraffin sections) (IHC-P). GTX636335
Anti-SDHA antibody [HL1117] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636335
Anti-SDHA antibody [HL1117] used in IHC (Paraffin sections) (IHC-P). GTX636335
Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335
Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335
Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335
Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335
Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335
Anti-SDHA antibody [HL1117] used in IHC (Whole mount) (IHC-Wm). GTX636335
Anti-SDHA antibody [HL1117] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636335
Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335

Cat. No. GTX636335

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1117

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all SDHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909973

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335

GTX636335 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1117] used in IHC (Paraffin sections) (IHC-P). GTX636335

GTX636335 IHC-P Image

SDHA antibody [HL1117] detects SDHA protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SDHA antibody [HL1117] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636335

GTX636335 ICC/IF Image

SDHA antibody [HL1117] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SDHA antibody [HL1117] used in IHC (Paraffin sections) (IHC-P). GTX636335

GTX636335 IHC-P Image

SDHA antibody [HL1117] detects SDHA protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335

GTX636335 WB Image

Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335

GTX636335 WB Image

Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335

GTX636335 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335

GTX636335 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335

GTX636335 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody [HL1117] used in IHC (Whole mount) (IHC-Wm). GTX636335

GTX636335 IHC-Wm Image

SDHA antibody [HL1117] detects SDHA protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SDHA antibody [HL1117] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636335

GTX636335 ICC/IF Image

Orthogonal

SDHA antibody [HL1117] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:500.
Red: MitoTracker® Red CMXRos, a mitochondria marker.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SDHA antibody [HL1117] used in Western Blot (WB). GTX636335

GTX636335 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations