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SDHA antibody

Anti-SDHA antibody used in Western Blot (WB). GTX101689
Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX101689
Anti-SDHA antibody used in Western Blot (WB). GTX101689
Anti-SDHA antibody used in Western Blot (WB). GTX101689
Anti-SDHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101689
Anti-SDHA antibody used in Western Blot (WB). GTX101689

Cat No. GTX101689

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , Molt-4 , mouse brain

Predict Reactivity

Zebrafish, Drosophila, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

succinate dehydrogenase complex flavoprotein subunit A,CMD1GG,FP,PGL5,SDH1,SDH2,SDHF

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody (GTX101689) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX101689

GTX101689 IHC-P Image

SDHA antibody detects SDHA protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
SDHA antibody (GTX101689) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101689 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

Sample (30 µg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX101689 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101689

GTX101689 ICC/IF Image

SDHA antibody detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA protein stained by SDHA antibody (GTX101689) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-SDHA antibody used in Western Blot (WB). GTX101689

GTX101689 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody (GTX101689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169