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SDHA antibody

Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX33490
Anti-SDHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33490
Anti-SDHA antibody used in Western Blot (WB). GTX33490
Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX33490
Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX33490

Cat. No. GTX33490

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all SDHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX33490

GTX33490 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX33490 SDHA antibody.
Dilution : 1:100

Anti-SDHA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33490

GTX33490 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33490 SDHA antibody.
Blue : DAPI

Anti-SDHA antibody used in Western Blot (WB). GTX33490

GTX33490 WB Image

WB analysis of various sample lysates using GTX33490 SDHA antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX33490

GTX33490 IHC-P Image

IHC-P analysis of rat heart tissue using GTX33490 SDHA antibody.
Dilution : 1:100

Anti-SDHA antibody used in IHC (Paraffin sections) (IHC-P). GTX33490

GTX33490 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX33490 SDHA antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419