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SDHAF1 antibody

Anti-SDHAF1 antibody used in Western Blot (WB). GTX17172
Anti-SDHAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17172
Anti-SDHAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17172

Cat. No. GTX17172

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all SDHAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

3T3 (NIH) Cell Lysate(GTX27179) , 3T3/BALB Cell Slide

Product Note

SDHAF1 antibody is predicted to not cross-react with other SDHAF protein family members.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SDHAF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SDHAF1.The immunogen is located within the last 50 amino acids of SDHAF1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate dehydrogenase complex assembly factor 1 , LYRM8

Cellular Localization

Mitochondrion matrix

Background

The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-SDHAF1 antibody used in Western Blot (WB). GTX17172

GTX17172 WB Image

WB analysis of 3T3 cell lysate using GTX17172 SDHAF1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-SDHAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17172

GTX17172 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX17172 SDHAF1 antibody.
Working concentration : 5 μg/ml

Anti-SDHAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17172

GTX17172 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX17172 SDHAF1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399