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SDHB antibody, C-term

Anti-SDHB antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89491
Anti-SDHB antibody, C-term used in Western Blot (WB). GTX89491

Cat No. GTX89491

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
IHC-P 5μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89491-PEP).

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

succinate dehydrogenase complex iron sulfur subunit B , CWS2 , IP , PGL4 , SDH , SDH1 , SDH2 , SDHIP

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SDHB antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89491

GTX89491 IHC-P Image

IHC-P analysis of human heart using GTX89491 SDHB antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-SDHB antibody, C-term used in Western Blot (WB). GTX89491

GTX89491 WB Image

WB analysis of human liver lysate using GTX89491 SDHB antibody, C-term.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf