GeneTex
United States (US)

SDHB antibody [C2C3], C-term

Anti-SDHB antibody [C2C3], C-term used in Western Blot (WB). GTX104628
Anti-SDHB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104628
Anti-SDHB antibody [C2C3], C-term used in Western Blot (WB). GTX104628
Anti-SDHB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104628
Anti-SDHB antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104628

Cat No. GTX104628

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

THP-1 , NCI-H929 , Neuro2A

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SDHB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Succinate Dehydrogenase Complex Iron Sulfur Subunit B,Cws2,Ip,Pgl4,Sdh,Sdh1,Sdh2,Sdhip,Sdhb

Cellular Localization

Mitochondrion inner membrane

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SDHB antibody [C2C3], C-term used in Western Blot (WB). GTX104628

GTX104628 WB Image

SDHB antibody [C2C3], C-term detects SDHB protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
12% SDS-PAGE
SDHB antibody [C2C3], C-term (GTX104628) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104628

GTX104628 IHC-P Image

SDHB antibody [C2C3], C-term detects SDHB protein at mitochondria on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody [C2C3], C-term used in Western Blot (WB). GTX104628

GTX104628 WB Image

SDHB antibody [C2C3], C-term detects SDHB protein by western blot analysis.
A. 30 µg THP-1 whole cell lysate/extract
B. 30 µg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
SDHB antibody [C2C3], C-term (GTX104628) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104628

GTX104628 IHC-P Image

SDHB antibody [C2C3], C-term detects SDHB protein at mitochindria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104628

GTX104628 ICC/IF Image

SDHB antibody [C2C3], C-term detects SDHB protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHB protein stained by SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169