SDHB antibody [C2C3], C-term

Name: SDHB antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX104628
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-SDHB antibody [C2C3], C-term used in Western Blot (WB). GTX104628

Anti-SDHB antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104628

Anti-SDHB antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104628

Cat. No. GTX104628

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all SDHB products

View product citations for antibody GTX104628 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

THP-1 , NCI-H929 , Neuro2A

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SDHB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241301

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate dehydrogenase complex iron sulfur subunit B , CWS2 , IP , PGL4 , SDH , SDH1 , SDH2 , SDHIP

Cellular Localization

Mitochondrion inner membrane

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Database

Gene ID: 6390 SDHB
UniProt: P21912 SDHB

Research Area

DATA IMAGES

GTX104628 WB Image

SDHB antibody [C2C3], C-term detects SDHB protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
12% SDS-PAGE
SDHB antibody [C2C3], C-term (GTX104628) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104628 IHC-P Image

SDHB antibody [C2C3], C-term detects SDHB protein at mitochindria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104628 ICC/IF Image

SDHB antibody [C2C3], C-term detects SDHB protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHB protein stained by SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX104628 IHC-P Image

SDHB antibody [C2C3], C-term detects SDHB protein at mitochondria on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
SDHB antibody [C2C3], C-term (GTX104628) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104628 WB Image

SDHB antibody [C2C3], C-term detects SDHB protein by western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
B. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
SDHB antibody [C2C3], C-term (GTX104628) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104628 WB Image

The data was published in the 2022 in Commun Biol. PMID: 35552531

GTX104628 WB Image

The data was published in the 2022 in Commun Biol. PMID: 35552531

REFERENCE

Application Reference

Takuro Toda et al. Commun Biol 2022; 5 (1) : 453 Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.

Application : WB

Sask?i ? et al. Dis Model Mech 2020; 13 (10) The SDHB Arg230His mutation causing familial paraganglioma alters glycolysis in a new Caenorhabditis elegans model.

Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4) : 744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer

Application : WB

Reactivity : Human

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