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SDHB antibody

Anti-SDHB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in Western Blot (WB). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in Western Blot (WB). GTX113833

Cat No. GTX113833

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

NCI-H929 , mouse brain

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SDHB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037951

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

succinate dehydrogenase complex iron sulfur subunit B , CWS2 , IP , PGL4 , SDH , SDH1 , SDH2 , SDHIP

Cellular Localization

Mitochondrion inner membrane

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SDHB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113833

GTX113833 ICC/IF Image

SDHB antibody detects SDHB protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in MeOH for 5 min.
Green: SDHB protein stained by SDHB antibody (GTX113833) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in Western Blot (WB). GTX113833

GTX113833 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113833 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in Western Blot (WB). GTX113833

GTX113833 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SDHB antibody (GTX113833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf