Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC13L1 antibody [N1C3] (GTX101055) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SEC13L1 antibody immunoprecipitates SEC13L1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 μg of SEC13L1 antibody (GTX101055) 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody (GTX101055) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

SEC13L1 antibody [N1C3] detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SEC13L1 protein stained by SEC13L1 antibody [N1C3] (GTX101055) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SEC13L1(GTX101055) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min