GeneTex
United States (US)

SEC13L1 antibody [N1C3]

GTX101055_WB_19011506_210.jpg
GTX101055_40093_IHC_19011506_931.jpg
GTX101055_40093_IFA_19011506_930.jpg
GTX101055_40093_IP_19011506_798.jpg

Cat No. GTX101055

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEC13L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sec13 Homolog, Nuclear Pore And Copii Coat Complex Component,D3S1231E,Sec13L1,Sec13R,Npp-20,Sec13

Background

The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq]

Database

DATA IMAGES
GTX101055_WB_19011506_210.jpg

GTX101055 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
10% SDS PAGE
GTX101055 diluted at 1:1000

GTX101055_40093_IHC_19011506_931.jpg

GTX101055 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SEC13L1(GTX101055) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101055_40093_IFA_19011506_930.jpg

GTX101055 ICC/IF Image

SEC13L1 antibody [N1C3] detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SEC13L1 protein stained by SEC13L1 antibody [N1C3] (GTX101055) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101055_40093_IP_19011506_798.jpg

GTX101055 IP Image

SEC13L1 antibody immunoprecipitates SEC13L1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 μg of SEC13L1 antibody (GTX101055) 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody (GTX101055) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169