GeneTex
United States (US)

SEC23IP antibody

GTX115597_41346_WB_18121410_277.jpg
GTX115597_41346_IFA_18121410_642.jpg
GTX115597_41346_IHC_M_18121410_993.jpg

Cat No. GTX115597

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

111 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEC23IP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sec23 Interacting Protein,Mstp053,P125,P125A,Sec23Ip

Cellular Localization

Cytoplasmic vesicle , COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side , Endoplasmic reticulum

Background

COPII-coated vesicles are involved in protein transport from the endoplasmic reticulum to the Golgi apparatus. The protein encoded by this gene was identified by its interaction with a mouse protein similar to yeast Sec23p, an essential component of the COPII. This protein shares significant similarity with phospholipid-modifying proteins, especially phosphatidic acid preferring-phospholipase A1. Overexpression of this protein has been shown to cause disorganization of the endoplasmic reticulum-Golgi intermediate compartment and Golgi apparatus, which suggests its role in the early secretory pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115597_41346_WB_18121410_277.jpg

GTX115597 WB Image

SEC23IP antibody detects SEC23IP protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg HepG2 whole cell lysate/extract
5 % SDS-PAGE
SEC23IP antibody (GTX115597) dilution: 1:500

GTX115597_41346_IFA_18121410_642.jpg

GTX115597 ICC/IF Image

SEC23IP antibody detects SEC23IP protein at cytoplasm by immunofluorescent analysis.
Sample: SKNSH cells were fixed in ice-cold MeOH for 5 min.
Green: SEC23IP protein stained by SEC23IP antibody (GTX115597) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115597_41346_IHC_M_18121410_993.jpg

GTX115597 IHC-P Image

SEC23IP antibody detects SEC23IP protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
SEC23IP antibody (GTX115597) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169