APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , HeLa , Jurkat , NT2D1
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.7mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SEC31A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ABP125 antibody, ABP130 antibody, DKFZp686N07171 antibody, HSPC334 antibody, KIAA0905 antibody, MGC90305 antibody, SEC31L1 antibody, SEC31A antibody, SEC31-related protein A antibody, protein transport protein Sec31A antibody, web1-like protein antibody, SEC31-like protein 1 antibody, yeast Sec31p homolog antibody, SEC31 homolog A (S. cerevisiae) antibody
Background
The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII , in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Database
DATA IMAGES
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GTX105784 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SEC31A(GTX105784) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX105784 WB Image
Sample (30 ug of whole cell lysate)
A: A549
B: Hela
5% SDS PAGE
GTX105784 diluted at 1:1000
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