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SEC31A antibody [N1N3]

Anti-SEC31A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105784
Anti-SEC31A antibody [N1N3] used in Western Blot (WB). GTX105784

Cat No. GTX105784

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

A549 , HeLa , Jurkat , NT2D1

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SEC31A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SEC31 homolog A, COPII coat complex component,ABP125,ABP130,HSPC275,HSPC334,SEC31L1

Background

The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII , in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

DATA IMAGES
Anti-SEC31A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105784

GTX105784 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SEC31A(GTX105784) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEC31A antibody [N1N3] used in Western Blot (WB). GTX105784

GTX105784 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hela
5% SDS PAGE
GTX105784 diluted at 1:1000

REFERENCE
There are currently no references for SEC31A antibody [N1N3] (GTX105784). Be the first to share your publications with this product.
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169