APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , HeLa , Jurkat , NT2D1
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SEC31A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037952
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
SEC31 homolog A, COPII coat complex component , ABP125 , ABP130 , HSPC275 , HSPC334 , SEC31L1
Background
The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII , in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-SEC31A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105784](/templates/default/js/lightbox/images/loading.gif "Anti-SEC31A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105784")
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GTX105784 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SEC31A(GTX105784) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX105784 WB Image
Sample (30 ug of whole cell lysate)
A: A549
B: Hela
5% SDS PAGE
GTX105784 diluted at 1:1000
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REFERENCE
There are currently no references for SEC31A antibody [N1N3] (GTX105784). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SEC31A antibody [N1N3] (GTX105784). Be the first to share your experience with this product.
![Anti-SEC31A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105784](/upload/website/prouct_img/small/GTX105784/GTX105784_39771_IHC_19011506_139.jpg "Anti-SEC31A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105784")
![Anti-SEC31A antibody [N1N3] used in Western Blot (WB). GTX105784](/upload/website/prouct_img/small/GTX105784/GTX105784_WB_19011506_870.jpg "Anti-SEC31A antibody [N1N3] used in Western Blot (WB). GTX105784")
![Anti-SEC31A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105784](/upload/website/prouct_img/normal/GTX105784/GTX105784_39771_IHC_19011506_139.jpg "Anti-SEC31A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX105784")
![Anti-SEC31A antibody [N1N3] used in Western Blot (WB). GTX105784](/upload/website/prouct_img/normal/GTX105784/GTX105784_WB_19011506_870.jpg "Anti-SEC31A antibody [N1N3] used in Western Blot (WB). GTX105784")